Microbial production of biosurfactants and their importance pdf

A major class of biosurfactants identified to be produced by bacteria are the glycolipids, however a number of bacteria have been shown to produce lipopeptides which have surface. Veenanadig pesticide residue abatement lab, food protectants and infestation control department, central food technological research institute. Pseudomonasderived biosurfactants are involved in many important bacterial functions. The chemically synthesized surfactants are usually classified according to their polarity, whereas, biosurfactants are generally categorized by their microbial origin and chemical composition as following. Production of bss by fermentation of fats, oils, and their coproducts has also been reported solaiman et al. The aim of this study was to investigate the production of biosurfactants by soil fungi isolated from the amazon forest. Recently, the interest for its biotechnological production has increased, because of their environme ntal, industrial, and pharmaceutical applications, and others.

After routine biosurfactant screening by various methods, the biosurfactant producing bacteria, halomonas sp bs4 was confirmed by 16 s rrna sequencing. Microbial biosurfactants role in oil products biodegradation. While the low molecular weight surfactants are often glycolipids, the high molecular weight surfactants are generally either polyanionic heteropolysaccharides containing covalentlylinked hydrophobic side chains or complexes containing both. They are carbohydrates linked to longchain aliphatic acids or hydroxyaliphatic acids by an ester group. Microbial production of biosurfactants sciencedirect. Microbial production of surfactants and their commercial. Biosurfactant producing microorganisms may play an important role in the accelerated bioremediation of hydrocarbon contaminated sites. Biosurfactant producing microbes and their potential. Biosurfactant production by microorganism for enhanced oil. Rhamnolipids are a class of biosurfactants with effective surfaceactive properties. These properties hold promise for biosurfactants to increase the degree of microbial destruction of petroleum hydrocarbons, which promotes the effective remediation of the petroleum pollution.

Our group has an interest in the microbial production of biosurfactants and their potential industrial or pharmacological applications 8. This results most often from the low concentration of biosurfactants in the fermentation medium and the expenses associated with fermentation and downstream processing. Microbial biosurfactants as additives for food industries. General surfactant structures according to the composition of their hydrophilic moieties and into nonionic, anionic, cationic, amphoteric compositions. Microbial production of biosurfactants from some elkorma. Thereason for their popularity, as high value microbial products, is primarily in their specific action, low toxicity, relative ease of preparation and widespread applicaility. Elsevier resources, conservation and recycling 18 1996 4157 mm, conservation andrecycung microbial production of biosurfactants m. Biosurfactants journal of petroleum and environmental. In this research a bacterial specie s with the ability of producing. Biosurfactantstypes, sources and applications request pdf.

Biosurfactants are surfaceactive compounds that have sparked interest in recent years because of their environmental advantages over conventional surfactants. The aim of the present study was production of biosurfactants from some bacterial isolates for bioremediation of crude oil spills. To begin with, relevant experiments have been performed only with a few emulsifier. Biosurfactant production by microorganism for enhanced oil recovery. Biosurfactants are amphiphilic biological compounds produced extracellularly or as part of the cell membrane by a variety of yeast, bacteria and filamentous fungi chen et al. Biosurfactants are capable of replacing chemically synthesized compounds in different areas of. Biosurfactants as useful tools in bioremediation intechopen.

Surfactants are chemically synthesized surfaceactive compounds widely used for large number of applications in various industries. From the above observation, it was concluded that both bacterial isolates of bacillus and pseudomonas have the ability to secrete surface active agents it is gain more importane in future for industrial and environmental applications references. Examples include their role in biodegradation and aid in swarming motility. Microbial biosurfactants today the global market for detergents in on the rise and the world wide surfactants consumption is expected to reach an approximate 10 billion kilogram by the year 2000. A large variety of microorganisms produce potent surfaceactive agents, biosurfactants, which vary in their chemical properties and molecular size. Microorganisms use a set of carbon sources and energy for growth. Their production, on the other hand, in many cases improves with increased salinity. A large variety of microorganisms produce potent surfaceactive agents, biosurfactants, which vary in their chemical properties. Microbial production of surfactants and their commercial potencial. Biosurfactants have also found utility in fields such as environmental bioremediation, food processing and pharmaceuticals. Biosurfactants are a group of microbial molecules identified by their unique capabilities to interact with hydrocarbons. Halophilic bacteria were isolated from thamaraikulam solar salt works in india.

Characterization of biosurfactants and their use in. Microbial biosurfactants attracted considerable attention because of their characteristics of biodegradability, low toxicity, ecological acceptability, and the ability to be produced figure 1. Biosurfactants enhance the emulsification of hydrocarbons, have the potential to solubilise hydrocarbon contaminants and increase their availability for microbial degradation. Pdf microbial production of biosurfactants and their. Orathai pornsunthorntawee, panya wongpanit, ratana rujiravanit. Enhanced biosurfactant production through cloning of three. Genes for surfactin production sfp, srfa, and coma in bacillus spp. Although the type and amount of the microbial surfactants produced depend primarily on the producer organism, factors like carbon and nitrogen, trace elements, temperature, and aeration also affect their production by the organism. Thus, they are the biosurfactants of choice for the venezuelan oil industry and in the cosmetics, food, and pharmaceutical markets. Murphy department of chemical engineering, the queens university of belfast, david keir building, stranmillis road, belfast, n. Towards commercial production of microbial surfactants.

Biosurfactants are classified according to their molecular weight and categorized by their microbial origin and composition. Genetic regulations of the biosynthesis of microbial surfactants. The combination of carbon sources with insoluble substrates facilitates the intracellular diffusion and production of different substances 10,11,12. The high molecular weight biosurfactants include the lipopolysaccharides but those of main interest are the low molecular weight glycolipids and lipopeptides lps and phospholipids. During last few years there is increase demand of biological surfaceactive compounds or biosurfactants which are produced by large number of microorganisms as they exert biodegradability, low toxicity and widespread application compared to chemical. Fermentative production of biosurfactants depends primarily on the microbial strain, source of carbon and nitrogen, ph, temperature, and concentration of oxygen and metal ions. Production of biosurfactants is a common feature in bacteria, and in particular in plantassociated species. Through microbial biodiversity exploration, on terrestrial or aquatic environments polluted with persistant organic compounds nonsoluble in water, is possible to isolate. Fungi colonies were isolated from soil samples and screened for biosurfactant production in submerged fermentation. Their observation suggests that cassava flour wastewater as a promising source of nutrients for bs production.

Genetic regulations of the biosynthesis of microbial. Great emphasis has recently been given to the environmental impacts caused by chemical surfactants due to their toxicity and difficulty in being degraded in the environment. The prospects of biosurfactants have a great potential because of their applications in the petroleum industry 1, 7, 8 and microbial enhanced oil recovery 914. Role of biosurfactants in nature and biotechnological. The success of biosurfactant production depends on the development of cheaper. Ireland, uk abstract biosurfactants are those chemicals which are produced by microorganisms but which. The rhodococcus ruber biosurfactants are found to be 1. An interesting example of a biosurfactant is the heptapeptide surfactin, a. Nadia krieger, doumit camilios neto, david alexander mitchell. Microbial biosurfactants production, applications and.

Microbial production of biosurfactants and their importance. Biosurfactants are the surfactants of microbial origin. The most significant limitation on the largescale production of biosurfactants and their widespread use is the high cost of their manufacturing. These bacteria include many plant beneficial and plant pathogenic pseudomonas spp. Biosurfactants have gained importance in the fields of enhanced oil recovery, environmental bioremediation, food processing and pharmaceuticals owing to their unique properties such as higher. Among the heterogeneous group of biosurfactants, the rhamnosecontaining glycolipids produced by pseudomonas arinos, 1996. Industrial production of microbial metabolites is a very complex process, and for industrial production, many variables are needed to be considered. Increasing environmental concerns, the advance in biotechnology and the emergence of more stringent laws have led to biosurfactants being a potential alternative to the chemical surfactants available on the market 2, 3. Biosurfactants have several applications in agriculture, industry, medicine and the petroleum sectors. Biosurfactants are a group of surface active agents which are produced by microorganisms. Biosurfactants in plantpseudomonas interactions and their. Addition of waterimmiscible substrates to media and nitrogen and iron limitations in the. Microorganisms yeasts, bacteria and some filamentous fungi are capable of producing biosurfactants with different molecular structures and.

Factors influencing biosurfactants production are the nature of the carbon source, nitrogen source, the c. Biosurfactants production, technology, applications. Biosurfactants or microbial surfactants are surfaceactive biomolecules that are produced by a variety of microorganisms. Microbial community composition of samples obtained from the phyllosphere of arabidopsis thaliana, soybean, clover and rice grown under environmental conditions 12,19. Biosurfactants are surface active amphipathic molecules produced by a plethora of microorganisms. Environmental importance of rhamnolipid production from. Production of biosurfactants by soil fungi isolated from. Biosurfactants have a widerange of applications due to their unique properties like specificity, low toxicity and relative ease of preparation. However, carbohydrates and vegetable oils are among the most widely used substrates for research on. The high cost of microbial production of rhamnolipids largely affects their commercial applications. Cost effective technologies and renewable substrates for. Screening optimization and production of biosurfactants.

Biosurfactants comprise a wide range of chemical structures, such as glycolipids, lipopeptides, polysaccharideprotein complexes, phospholipids, fatty acids and neutral lipids. Elyas golabi 1, seyed ruhollah mortazavi poor sogh 1, seyed noroldin hosseini 1, mohammad amin gholamzadeh 1. Production of microbial biosurfactants by solidstate cultivation. The emergence of microbial biosurfactants and their. Paper open access factors affecting the production of. Production and their potential in environmental biotechnology. Bacterial biosurfactants, and their role in microbial. Biosurfactants microbial origin bacteria fungi rhamnolipid p. Biosurfactants microbial surface active agents have become recently an important product of biotechnology for industrial and medical applications. They offer so many advantages over their synthetic counterparts due to their biodegradable and environmental friendly nature, thats why gaining much more attention in creating the era of green technology. Microbial compounds that exhibit pronounced surface and emulsifying activities are classified as biosurfactants.

Although bioemulsifiers are produced by a large number of microorganisms and are clearly significant in many aspects of growth, it is difficult to generalize on their roles in microbial physiology. Twenty five bacterial isolates were isolated from twenty crude oil contaminated soils. The importance of biosurfactants, their production, characteristics and limited successes and applications in oil pollution remediation and oil storage tank cleaning are discussed. Microbial production of biosurfactants and their importance n. The nitrogen source can be an important key to the regulation of bs synthesis. The growth optimization of halomonas sp bs4 revealed their optimum growth at 8% nacl and 68 ph in the growth medium.